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KMID : 0665220170300061279
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Korean Journal of Food and Nutrition
2017 Volume.30 No. 6 p.1279 ~ p.1285
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Protective Effect of PineXol® against Amyloid-β-induced Cell Death
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Han Kyung-Hoon
Lee Seung-Hee
Park Kwang-Sung
Song Kwan-Young
Kim Jung-Hee
Park Eun-Kuk
Han Sung-Hee
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Abstract
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Amyloid-β protein (Aβ) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of PineXol® on Aβ25-35 induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with 100 μg/mL of PineXol® for 2 h. The cells were exposed to single dose of 30 μM Aβ25-35 for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both Aβ25-35 and PineXol®, compared to the control group. PineXol® induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of Aβ25-35 induced apoptosis. These results suggest that PineXol® may be a good candidate for the prevention of Alzheimer’s disease(AD).
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KEYWORD
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PineXol®, Alzheimer’s disease, amyloid-β, PC-12 cells, Bcl-2
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